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Inova Diagnostics hep 2 cells
Hep 2 Cells, supplied by Inova Diagnostics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hep-2+cells/2+cells+hep/pmc13260532-72-16-18
Average 86 stars, based on 1 article reviews
hep 2 cells - by Bioz Stars, 2026-10
86/100 stars

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Immunofluorescence:

Article Title: Unique Serum Immune Phenotypes and Stratification of Oklahoma Native American Rheumatic Disease Patients.
Article Snippet: Antinuclear antibodies (ANAs) were determined by indirect immunofluorescence with HEp-2 cells (INOVA Diagnostics).

Article Title: Biomarkers for assessing risk of transitioning to systemic lupus erythematosus classification and disease pathogenesis
Article Snippet: Briefly, ANAs (detected using HEp-2 cells) and anti-double-stranded DNA (anti-dsDNA) antibodies (determined using Crithidia luciliae assays) were measured in the serum using IIF (Inova Diagnostics); seropositivity for ANAs was defined as a titer of ≥1:120, and seropositivity for anti-dsDNA was defined as a titer of ≥1:30.

Article Title: Inactivated SARS-CoV-2 vaccine in primary Sjögren’s syndrome: humoral response, safety, and effects on disease activity
Article Snippet: The three serum samples from each patient/control (collected on D0, D28, and D69) were assayed for antinuclear antibodies (ANA) by indirect immunofluorescence on HEp-2 cells (INOVA Diagnostics Inc., San Diego, USA), anti-Ro(SS-A), and anti-La(SS-B) (INOVA Diagnostics Inc., San Diego, USA), following the recommendations of the manufacturer.

Article Title: Mild-to-moderate COVID-19 does not predispose to the development of autoimmune rheumatic diseases or autoimmune-based thrombosis.
Article Snippet: A high number of respiratory tract infections with a novel coronavirus was observed in December 2019 in Wuhan, China.. The new pathogen called severe acute respiratory syndrome coronavirus, has spread quickly around the world, forcing the World Health Organization (WHO) to declare the coronavirus disease 2019 pandemic in May 2020.1 The clinical symptoms of SARSCoV2 infection may vary from mild ones, such as fever, cough, myalgia, headache etc., to lifethreatening systemic conditions, for example, disseminated intravascular coagulation (DIC), pneumonia, acute respiratory distress syndrome (ARDS) and hyperinflammation, which may have a negative impact on many vital organs and systems, including the renal, cardiovascular and nervous systems, as well as on haematopoiesis.2,3 There are many lines of evidence supporting the thesis that interactions between the viral and host Received: 26 April 2023 | Accepted: 16 July 2023 DOI: 10.1111/sji.13313

Article Title: Update of the simplified criteria for autoimmune hepatitis: Evaluation of the methodology for immunoserological testing.
Article Snippet: 79 80 Background & Aims: The simplified criteria for the diagnosis of autoimmune 81 hepatitis (AIH) include immunofluorescence testing (IFT) of antinuclear and smooth 82 muscle autoantibodies (ANA and SMA) on rodent tissue sections.. We aimed to 83 establish scoring criteria for implementation of ANA IFT on HEp-2 cells and ELISA84 based testing.. Methods: ANA and SMA reactivity of 61 AIH sera and 72 non85 alcoholic fatty liver disease (NAFLD) controls were separately assessed on tissue 86 sections and human epithelioma (HEp-2) cells to compare the diagnostic value at 87 increasing titers.

Article Title: Autoantibody-positive healthy individuals with lower lupus risk display a unique immune endotype
Article Snippet: Positive individuals were also assessed by NOVA Lite indirect immunofluorescence (IIF) and QUANTA Lite ELISA using HEP-2 cells (Inova Diagnostics, Inc., San Diego, CA) following the manufacturer’s recommended protocols and cutoffs ( 7 , 16 , 17 ).

Article Title: Comparison of pulmonary and small airways function between idiopathic inflammatory myopathies patients with and without interstitial lung disease.
Article Snippet: Immunological work-up included indirect immunofluorescence for antinuclear antibodies (ANA) on commercially available Hep-2 cells and ethanolfixed neutrophils using the NOVA Lite HEp-2 ANA kit (Inova Diagnostics Inc, San Diego, CA, USA), according to manufacturer’s instructions, and immunoblotting for extractable nuclear antigen antibodies (ENA), including anti-Ro, anti-La, anti-U1 ribonucleoprotein (anti-U1RNP) and anti-Sm antibodies, using the Euroline Anti-ENA ProfilePlus1 (IgG) kit (Euroimmun, Lübeck, Germany).

Article Title: Comparisons of Anti-dsDNA Antibody Detection Methods by Chemiluminescent Immunoassay and Enzyme-Linked Immunosorbent Assay in Systemic Lupus Erythematosus
Article Snippet: ANA was detected using indirect immunofluorescence assay on Hep-2 cells (Inova Diagnostics); titer was detected with the NOVA View automated fluorescence microscope; a titer of ≥1:160 was considered positive and ≥1:640 was considered strongly positive.

Western Blot:

Article Title: Unique Serum Immune Phenotypes and Stratification of Oklahoma Native American Rheumatic Disease Patients.
Article Snippet: Antinuclear antibodies (ANAs) were determined by indirect immunofluorescence with HEp-2 cells (INOVA Diagnostics).

Article Title: Biomarkers for assessing risk of transitioning to systemic lupus erythematosus classification and disease pathogenesis
Article Snippet: Briefly, ANAs (detected using HEp-2 cells) and anti-double-stranded DNA (anti-dsDNA) antibodies (determined using Crithidia luciliae assays) were measured in the serum using IIF (Inova Diagnostics); seropositivity for ANAs was defined as a titer of ≥1:120, and seropositivity for anti-dsDNA was defined as a titer of ≥1:30.

Article Title: Inactivated SARS-CoV-2 vaccine in primary Sjögren’s syndrome: humoral response, safety, and effects on disease activity
Article Snippet: The three serum samples from each patient/control (collected on D0, D28, and D69) were assayed for antinuclear antibodies (ANA) by indirect immunofluorescence on HEp-2 cells (INOVA Diagnostics Inc., San Diego, USA), anti-Ro(SS-A), and anti-La(SS-B) (INOVA Diagnostics Inc., San Diego, USA), following the recommendations of the manufacturer.

Article Title: Mild-to-moderate COVID-19 does not predispose to the development of autoimmune rheumatic diseases or autoimmune-based thrombosis.
Article Snippet: A high number of respiratory tract infections with a novel coronavirus was observed in December 2019 in Wuhan, China.. The new pathogen called severe acute respiratory syndrome coronavirus, has spread quickly around the world, forcing the World Health Organization (WHO) to declare the coronavirus disease 2019 pandemic in May 2020.1 The clinical symptoms of SARSCoV2 infection may vary from mild ones, such as fever, cough, myalgia, headache etc., to lifethreatening systemic conditions, for example, disseminated intravascular coagulation (DIC), pneumonia, acute respiratory distress syndrome (ARDS) and hyperinflammation, which may have a negative impact on many vital organs and systems, including the renal, cardiovascular and nervous systems, as well as on haematopoiesis.2,3 There are many lines of evidence supporting the thesis that interactions between the viral and host Received: 26 April 2023 | Accepted: 16 July 2023 DOI: 10.1111/sji.13313

Article Title: Update of the simplified criteria for autoimmune hepatitis: Evaluation of the methodology for immunoserological testing.
Article Snippet: 79 80 Background & Aims: The simplified criteria for the diagnosis of autoimmune 81 hepatitis (AIH) include immunofluorescence testing (IFT) of antinuclear and smooth 82 muscle autoantibodies (ANA and SMA) on rodent tissue sections.. We aimed to 83 establish scoring criteria for implementation of ANA IFT on HEp-2 cells and ELISA84 based testing.. Methods: ANA and SMA reactivity of 61 AIH sera and 72 non85 alcoholic fatty liver disease (NAFLD) controls were separately assessed on tissue 86 sections and human epithelioma (HEp-2) cells to compare the diagnostic value at 87 increasing titers.

Article Title: Autoantibody-positive healthy individuals with lower lupus risk display a unique immune endotype
Article Snippet: Positive individuals were also assessed by NOVA Lite indirect immunofluorescence (IIF) and QUANTA Lite ELISA using HEP-2 cells (Inova Diagnostics, Inc., San Diego, CA) following the manufacturer’s recommended protocols and cutoffs ( 7 , 16 , 17 ).

Article Title: Comparison of pulmonary and small airways function between idiopathic inflammatory myopathies patients with and without interstitial lung disease.
Article Snippet: Immunological work-up included indirect immunofluorescence for antinuclear antibodies (ANA) on commercially available Hep-2 cells and ethanolfixed neutrophils using the NOVA Lite HEp-2 ANA kit (Inova Diagnostics Inc, San Diego, CA, USA), according to manufacturer’s instructions, and immunoblotting for extractable nuclear antigen antibodies (ENA), including anti-Ro, anti-La, anti-U1 ribonucleoprotein (anti-U1RNP) and anti-Sm antibodies, using the Euroline Anti-ENA ProfilePlus1 (IgG) kit (Euroimmun, Lübeck, Germany).

Article Title: Comparisons of Anti-dsDNA Antibody Detection Methods by Chemiluminescent Immunoassay and Enzyme-Linked Immunosorbent Assay in Systemic Lupus Erythematosus
Article Snippet: ANA was detected using indirect immunofluorescence assay on Hep-2 cells (Inova Diagnostics); titer was detected with the NOVA View automated fluorescence microscope; a titer of ≥1:160 was considered positive and ≥1:640 was considered strongly positive.

Enzyme-linked Immunosorbent Assay:

Article Title: Unique Serum Immune Phenotypes and Stratification of Oklahoma Native American Rheumatic Disease Patients.
Article Snippet: Antinuclear antibodies (ANAs) were determined by indirect immunofluorescence with HEp-2 cells (INOVA Diagnostics).

Article Title: Biomarkers for assessing risk of transitioning to systemic lupus erythematosus classification and disease pathogenesis
Article Snippet: Briefly, ANAs (detected using HEp-2 cells) and anti-double-stranded DNA (anti-dsDNA) antibodies (determined using Crithidia luciliae assays) were measured in the serum using IIF (Inova Diagnostics); seropositivity for ANAs was defined as a titer of ≥1:120, and seropositivity for anti-dsDNA was defined as a titer of ≥1:30.

Article Title: Inactivated SARS-CoV-2 vaccine in primary Sjögren’s syndrome: humoral response, safety, and effects on disease activity
Article Snippet: The three serum samples from each patient/control (collected on D0, D28, and D69) were assayed for antinuclear antibodies (ANA) by indirect immunofluorescence on HEp-2 cells (INOVA Diagnostics Inc., San Diego, USA), anti-Ro(SS-A), and anti-La(SS-B) (INOVA Diagnostics Inc., San Diego, USA), following the recommendations of the manufacturer.

Article Title: Mild-to-moderate COVID-19 does not predispose to the development of autoimmune rheumatic diseases or autoimmune-based thrombosis.
Article Snippet: A high number of respiratory tract infections with a novel coronavirus was observed in December 2019 in Wuhan, China.. The new pathogen called severe acute respiratory syndrome coronavirus, has spread quickly around the world, forcing the World Health Organization (WHO) to declare the coronavirus disease 2019 pandemic in May 2020.1 The clinical symptoms of SARSCoV2 infection may vary from mild ones, such as fever, cough, myalgia, headache etc., to lifethreatening systemic conditions, for example, disseminated intravascular coagulation (DIC), pneumonia, acute respiratory distress syndrome (ARDS) and hyperinflammation, which may have a negative impact on many vital organs and systems, including the renal, cardiovascular and nervous systems, as well as on haematopoiesis.2,3 There are many lines of evidence supporting the thesis that interactions between the viral and host Received: 26 April 2023 | Accepted: 16 July 2023 DOI: 10.1111/sji.13313

Article Title: Update of the simplified criteria for autoimmune hepatitis: Evaluation of the methodology for immunoserological testing.
Article Snippet: 79 80 Background & Aims: The simplified criteria for the diagnosis of autoimmune 81 hepatitis (AIH) include immunofluorescence testing (IFT) of antinuclear and smooth 82 muscle autoantibodies (ANA and SMA) on rodent tissue sections.. We aimed to 83 establish scoring criteria for implementation of ANA IFT on HEp-2 cells and ELISA84 based testing.. Methods: ANA and SMA reactivity of 61 AIH sera and 72 non85 alcoholic fatty liver disease (NAFLD) controls were separately assessed on tissue 86 sections and human epithelioma (HEp-2) cells to compare the diagnostic value at 87 increasing titers.

Article Title: Autoantibody-positive healthy individuals with lower lupus risk display a unique immune endotype
Article Snippet: Positive individuals were also assessed by NOVA Lite indirect immunofluorescence (IIF) and QUANTA Lite ELISA using HEP-2 cells (Inova Diagnostics, Inc., San Diego, CA) following the manufacturer’s recommended protocols and cutoffs ( 7 , 16 , 17 ).

Article Title: Comparison of pulmonary and small airways function between idiopathic inflammatory myopathies patients with and without interstitial lung disease.
Article Snippet: Immunological work-up included indirect immunofluorescence for antinuclear antibodies (ANA) on commercially available Hep-2 cells and ethanolfixed neutrophils using the NOVA Lite HEp-2 ANA kit (Inova Diagnostics Inc, San Diego, CA, USA), according to manufacturer’s instructions, and immunoblotting for extractable nuclear antigen antibodies (ENA), including anti-Ro, anti-La, anti-U1 ribonucleoprotein (anti-U1RNP) and anti-Sm antibodies, using the Euroline Anti-ENA ProfilePlus1 (IgG) kit (Euroimmun, Lübeck, Germany).

Article Title: Comparisons of Anti-dsDNA Antibody Detection Methods by Chemiluminescent Immunoassay and Enzyme-Linked Immunosorbent Assay in Systemic Lupus Erythematosus
Article Snippet: ANA was detected using indirect immunofluorescence assay on Hep-2 cells (Inova Diagnostics); titer was detected with the NOVA View automated fluorescence microscope; a titer of ≥1:160 was considered positive and ≥1:640 was considered strongly positive.

Fluorescence:

Article Title: Unique Serum Immune Phenotypes and Stratification of Oklahoma Native American Rheumatic Disease Patients.
Article Snippet: Antinuclear antibodies (ANAs) were determined by indirect immunofluorescence with HEp-2 cells (INOVA Diagnostics).

Article Title: Biomarkers for assessing risk of transitioning to systemic lupus erythematosus classification and disease pathogenesis
Article Snippet: Briefly, ANAs (detected using HEp-2 cells) and anti-double-stranded DNA (anti-dsDNA) antibodies (determined using Crithidia luciliae assays) were measured in the serum using IIF (Inova Diagnostics); seropositivity for ANAs was defined as a titer of ≥1:120, and seropositivity for anti-dsDNA was defined as a titer of ≥1:30.

Article Title: Inactivated SARS-CoV-2 vaccine in primary Sjögren’s syndrome: humoral response, safety, and effects on disease activity
Article Snippet: The three serum samples from each patient/control (collected on D0, D28, and D69) were assayed for antinuclear antibodies (ANA) by indirect immunofluorescence on HEp-2 cells (INOVA Diagnostics Inc., San Diego, USA), anti-Ro(SS-A), and anti-La(SS-B) (INOVA Diagnostics Inc., San Diego, USA), following the recommendations of the manufacturer.

Article Title: Mild-to-moderate COVID-19 does not predispose to the development of autoimmune rheumatic diseases or autoimmune-based thrombosis.
Article Snippet: A high number of respiratory tract infections with a novel coronavirus was observed in December 2019 in Wuhan, China.. The new pathogen called severe acute respiratory syndrome coronavirus, has spread quickly around the world, forcing the World Health Organization (WHO) to declare the coronavirus disease 2019 pandemic in May 2020.1 The clinical symptoms of SARSCoV2 infection may vary from mild ones, such as fever, cough, myalgia, headache etc., to lifethreatening systemic conditions, for example, disseminated intravascular coagulation (DIC), pneumonia, acute respiratory distress syndrome (ARDS) and hyperinflammation, which may have a negative impact on many vital organs and systems, including the renal, cardiovascular and nervous systems, as well as on haematopoiesis.2,3 There are many lines of evidence supporting the thesis that interactions between the viral and host Received: 26 April 2023 | Accepted: 16 July 2023 DOI: 10.1111/sji.13313

Article Title: Update of the simplified criteria for autoimmune hepatitis: Evaluation of the methodology for immunoserological testing.
Article Snippet: 79 80 Background & Aims: The simplified criteria for the diagnosis of autoimmune 81 hepatitis (AIH) include immunofluorescence testing (IFT) of antinuclear and smooth 82 muscle autoantibodies (ANA and SMA) on rodent tissue sections.. We aimed to 83 establish scoring criteria for implementation of ANA IFT on HEp-2 cells and ELISA84 based testing.. Methods: ANA and SMA reactivity of 61 AIH sera and 72 non85 alcoholic fatty liver disease (NAFLD) controls were separately assessed on tissue 86 sections and human epithelioma (HEp-2) cells to compare the diagnostic value at 87 increasing titers.

Article Title: Autoantibody-positive healthy individuals with lower lupus risk display a unique immune endotype
Article Snippet: Positive individuals were also assessed by NOVA Lite indirect immunofluorescence (IIF) and QUANTA Lite ELISA using HEP-2 cells (Inova Diagnostics, Inc., San Diego, CA) following the manufacturer’s recommended protocols and cutoffs ( 7 , 16 , 17 ).

Article Title: Comparison of pulmonary and small airways function between idiopathic inflammatory myopathies patients with and without interstitial lung disease.
Article Snippet: Immunological work-up included indirect immunofluorescence for antinuclear antibodies (ANA) on commercially available Hep-2 cells and ethanolfixed neutrophils using the NOVA Lite HEp-2 ANA kit (Inova Diagnostics Inc, San Diego, CA, USA), according to manufacturer’s instructions, and immunoblotting for extractable nuclear antigen antibodies (ENA), including anti-Ro, anti-La, anti-U1 ribonucleoprotein (anti-U1RNP) and anti-Sm antibodies, using the Euroline Anti-ENA ProfilePlus1 (IgG) kit (Euroimmun, Lübeck, Germany).

Article Title: Comparisons of Anti-dsDNA Antibody Detection Methods by Chemiluminescent Immunoassay and Enzyme-Linked Immunosorbent Assay in Systemic Lupus Erythematosus
Article Snippet: ANA was detected using indirect immunofluorescence assay on Hep-2 cells (Inova Diagnostics); titer was detected with the NOVA View automated fluorescence microscope; a titer of ≥1:160 was considered positive and ≥1:640 was considered strongly positive.

Microscopy:

Article Title: Unique Serum Immune Phenotypes and Stratification of Oklahoma Native American Rheumatic Disease Patients.
Article Snippet: Antinuclear antibodies (ANAs) were determined by indirect immunofluorescence with HEp-2 cells (INOVA Diagnostics).

Article Title: Biomarkers for assessing risk of transitioning to systemic lupus erythematosus classification and disease pathogenesis
Article Snippet: Briefly, ANAs (detected using HEp-2 cells) and anti-double-stranded DNA (anti-dsDNA) antibodies (determined using Crithidia luciliae assays) were measured in the serum using IIF (Inova Diagnostics); seropositivity for ANAs was defined as a titer of ≥1:120, and seropositivity for anti-dsDNA was defined as a titer of ≥1:30.

Article Title: Inactivated SARS-CoV-2 vaccine in primary Sjögren’s syndrome: humoral response, safety, and effects on disease activity
Article Snippet: The three serum samples from each patient/control (collected on D0, D28, and D69) were assayed for antinuclear antibodies (ANA) by indirect immunofluorescence on HEp-2 cells (INOVA Diagnostics Inc., San Diego, USA), anti-Ro(SS-A), and anti-La(SS-B) (INOVA Diagnostics Inc., San Diego, USA), following the recommendations of the manufacturer.

Article Title: Mild-to-moderate COVID-19 does not predispose to the development of autoimmune rheumatic diseases or autoimmune-based thrombosis.
Article Snippet: A high number of respiratory tract infections with a novel coronavirus was observed in December 2019 in Wuhan, China.. The new pathogen called severe acute respiratory syndrome coronavirus, has spread quickly around the world, forcing the World Health Organization (WHO) to declare the coronavirus disease 2019 pandemic in May 2020.1 The clinical symptoms of SARSCoV2 infection may vary from mild ones, such as fever, cough, myalgia, headache etc., to lifethreatening systemic conditions, for example, disseminated intravascular coagulation (DIC), pneumonia, acute respiratory distress syndrome (ARDS) and hyperinflammation, which may have a negative impact on many vital organs and systems, including the renal, cardiovascular and nervous systems, as well as on haematopoiesis.2,3 There are many lines of evidence supporting the thesis that interactions between the viral and host Received: 26 April 2023 | Accepted: 16 July 2023 DOI: 10.1111/sji.13313

Article Title: Update of the simplified criteria for autoimmune hepatitis: Evaluation of the methodology for immunoserological testing.
Article Snippet: 79 80 Background & Aims: The simplified criteria for the diagnosis of autoimmune 81 hepatitis (AIH) include immunofluorescence testing (IFT) of antinuclear and smooth 82 muscle autoantibodies (ANA and SMA) on rodent tissue sections.. We aimed to 83 establish scoring criteria for implementation of ANA IFT on HEp-2 cells and ELISA84 based testing.. Methods: ANA and SMA reactivity of 61 AIH sera and 72 non85 alcoholic fatty liver disease (NAFLD) controls were separately assessed on tissue 86 sections and human epithelioma (HEp-2) cells to compare the diagnostic value at 87 increasing titers.

Article Title: Autoantibody-positive healthy individuals with lower lupus risk display a unique immune endotype
Article Snippet: Positive individuals were also assessed by NOVA Lite indirect immunofluorescence (IIF) and QUANTA Lite ELISA using HEP-2 cells (Inova Diagnostics, Inc., San Diego, CA) following the manufacturer’s recommended protocols and cutoffs ( 7 , 16 , 17 ).

Article Title: Comparison of pulmonary and small airways function between idiopathic inflammatory myopathies patients with and without interstitial lung disease.
Article Snippet: Immunological work-up included indirect immunofluorescence for antinuclear antibodies (ANA) on commercially available Hep-2 cells and ethanolfixed neutrophils using the NOVA Lite HEp-2 ANA kit (Inova Diagnostics Inc, San Diego, CA, USA), according to manufacturer’s instructions, and immunoblotting for extractable nuclear antigen antibodies (ENA), including anti-Ro, anti-La, anti-U1 ribonucleoprotein (anti-U1RNP) and anti-Sm antibodies, using the Euroline Anti-ENA ProfilePlus1 (IgG) kit (Euroimmun, Lübeck, Germany).

Article Title: Comparisons of Anti-dsDNA Antibody Detection Methods by Chemiluminescent Immunoassay and Enzyme-Linked Immunosorbent Assay in Systemic Lupus Erythematosus
Article Snippet: ANA was detected using indirect immunofluorescence assay on Hep-2 cells (Inova Diagnostics); titer was detected with the NOVA View automated fluorescence microscope; a titer of ≥1:160 was considered positive and ≥1:640 was considered strongly positive.



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